Hi there @benjaminbrooker
Just a kind reminder first, please keep in mind that it's important to specify the following when reporting an issue, that will help forums members & support to troubleshoot your problem & provide you with an answer.
OS + Version : i.e -> MacOS 10.13.6 / Win 10-1903
Plugin Version : i.e -> ANA 2 v2.0.92
DAW Version : i.e -> Live Suite 10.1.1
Platform & Plugin format used : i.e -> Using the 32 or 64 bit version of the Plugin, issue happening with the AAX, AU, VST plugin or with all of them.
Now back to this one, if you're on the latest version of ANA 2 which is v2.0.92, this behavior in Live when reloading an existing project is the current one, many other 3thrd party plugins behave in the same way where automation lines would be disabled when reopening a project. It's just a matter of re-enabling automation by clicking on the orange button in Live's Transport menu bar.
You just need to do this once and unless you manually change an already automated parameter in ANA 2, automation will remain active. In version 2.0.91 and before, all automations would be turned off when tweaking any parameter which was more of a problematic behavior.
So make sure to use the latest v2.0.92 version of ANA 2 ( if you're using ANA 2 via the Slate All Access Pass, please note that it will still be in v2.0.91 and therefore you may experience the unwanted behavior as explain above ).